In recent years, a new paradigm of nanobiosensors combining miniaturization and integration has been exploited in areas such as combinational chemistry, biotechnology, functional genomics, proteomics and clinical diagnostics. The new intelligent analysis systems with high signal-to-noise ratios, self-organization and various plate formats allow for automated high-throughput screening (HTS), drug delivery systems and single molecule analysis. The notion of automation, miniaturization and integration requires the fabrication of appropriately designed nanometrics for high sensitivity homogenous assays. In this paper, we present the nanometrics geometry of a well-oriented nanowell (ONW) array derived from nanofabrication technology which can easily be employed for digital detection with a high S/N ratio, miniaturization, integrated assays and single molecule analysis. In this geometry, most of the area of the Au electrode was covered with the blocking layer, and only a nanosized gold surface becomes exposed to the open space above the ONW.(1)-(3) We also present a strong specific antibody-antigen interaction on a functional lipid-membrane vesicle (liposome, FLVs) modified gold surface using ONW array electrode. The interaction is described by electrochemical measurerment, SPR, QCM, AFM. Additionally, we present simple soft lithographic methods for patterning supported lipid bilayer membranes onto a surface and inside microfluidic channels. (4) The patterned PEG surfaces resisted nonspecific adsorption of lipid vesicles allowing for adsorption of the lipid bilayers on the exposed regions of two dimensional surfaces and inside microfluidic channels in comparison to glass control. We believe these findings can be related to various liposome applications such as drug delivery system, electrochemical or biosensors and nano scale membrane function studies.
References:
1. H.Y.Lee, J.W.Park, J.M.Kim, H.S.Jung, and T.Kawai,, Appl. Phys. Lett., 89 (2006) 113901-113903.
2. J.M. Kim, H.S. Jung, J.W. Park, H.Oka, T.Yukimasa, H.Y. Lee, T.Kawai, J. Am. Chem. Soc., 127 (2005) 2358-2362
3. H.S. Jung, J.M. Kim, J.W. Park, H.Y. Lee, T.Kawai, Langmuir, 21 (2005) 6025-6029.
4. P.N.Kim, S.E.Lee, H.S.Jung, H. Y.Lee, T.Kawai, and K.Y. Suh. Lab on a chip, 6 (2006) 54-59.
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